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Hair loss is a problem that causes many irritations on the scalp and hair. One of the treatments for hair loss is using a natural hair tonic. Spinach leaves are used as hair growers because they contain protein (amino acids) and vitamins (A, B, C, and E) and minerals (Zn, Fe, and Ca). This study aims to determine the activity of spinach leaf ethanol extract cream preparation and the optimum concentration of Spinach leaf ethanol extract cream preparation for hair grower. This research is purely experimental with a completely randomized design with unidirectional pattern. Spinach leaf ethanol extract at concentrations of 20% w/v, 30% w/v, and 40% w/v is formulated in the form of cream type O/W with vanishing cream as the base ingredient. Observations were made by calculating the hair growth rate in rabbits. Hair growth activity data obtained in this study were calculated using the AGD (Average Growth Daily gain) formula and then statistically analyzed using the Analysis of variance (ANOVA) method with a confidence level of 95%. Spinach leaf ethanol extract cream at concentrations of 20% w/v, 30% w/v, and 40% w/v show hair growth activity. The best hair growth activity was shown by the ethanol extract of Spinach leaves at a concentration of 40% w/v.